Spatial single-cell analysis of colorectal cancer tumour using multiplexed imaging mass cytometry
Published in Journal for ImmunoTherapy of Cancer, 2020
Recommended citation: Tran M, Su A, Lee H, et (2020). "Spatial single-cell analysis of colorectal cancer tumour using multiplexed imaging mass cytometry" Journal for ImmunoTherapy of Cancer. 665. https://jitc.bmj.com/content/8/Suppl_3/A399.1
Authors: Tran, Minh and Su, Andrew and Lee, HoJoon and Cruz, Richard and Pflieger, Lance and Dean, Ashely and Nguyen, Quan and Ji, Hanlee and Rhodes, Terence
Abstract: Cancer research experiments often require the dissociation of cells from their native tissue before molecular profiling, leading to the loss of spatial tissue context. The cancer genomics research has shifted from mostly profiling tumour DNA mutations towards the current frontier of investigating individual genes and gene products in single cells and their immediate microenvironments. Information at this level with the spatial context enables us to link cancer–causing mutations and environmental factors to outcomes in cell signalling, responses and survival that will lead to solutions for diagnosing, predicting progression and treating cancers in different individuals. In this project we aim to capture tissue morphology, cancer cell types, multi-parameter protein contents of single cells in within morphologically intact tissue sections of colorectal tumours from 52 patients.